Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization

Journal of Biomolecular NMR, 2015, DOI 10.1007/s10858-015-9902-2, Volume 61, Issue 2, pp 151-160 published on 30.01.2015
Journal of Biomolecular NMR, online article
In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay..

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry