Latest News



leonhardt_2020_nishiyama_natcomm_550.100x0.jpg

Stable inheritance of DNA methylation is critical for maintaining differentiated phenotypes in multicellular organisms. We have recently identified dual mono-ubiquitylation of histone H3 (H3Ub2) by UHRF1 as an essential mechanism to recruit DNMT1 to chromatin. Here, we show that PCNA-associated factor 15 (PAF15) undergoes UHRF1-dependent dual mono-ubiquitylation ...

|READ MORE|

2020_schneider_plosone_550.100x0.jpg

The multi-domain protein UHRF1 is essential for DNA methylation maintenance and binds DNA via a base-flipping mechanism with a preference for hemi-methylated CpG sites. We investigated its binding to hemi- and symmetrically modified DNA containing either 5-methylcytosine (mC), 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), or 5-carboxylcytosine (caC). Our ...

|READ MORE|

cipsm_bayer_exk_2020_550.100x0.jpg

Excursion of students from the CIPSM graduate educational program to the Bayer AG as part of the lecture series on Innovations Management of Bayer AGs former Board of Management Member Wolfgang Plischke.

Please register here:  postponed indefinitely due to the current situation

|READ MORE|

leonhardt-conradt-2020_deng_natcomm_550.100x0.jpg

Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We ...

|READ MORE|

kickkameooilnoph.100x0.gif

 

It’s Christmas and we at CIPSM just wanted to say
Thank you and that it’s been a pleasure working with you this past year.
We wish you and your family wonderful holidays and a Happy New Year!

 

|READ MORE|

kellner-jung-reichle_2019_natcom_550.100x0.jpeg

RNAs contain post-transcriptional modifications, which fulfill a variety of functions in translation, secondary structure stabilization and cellular stress survival. Here, 2-methylthiocytidine (ms2C) is identified in tRNA of E. coli and P. aeruginosa using NAIL-MS (nucleic acid isotope labeling coupled mass spectrometry) in combination with genetic screening ...

|READ MORE|

carell-hornung-cell_2019_550.100x0.jpg

TLR8 is among the highest-expressed pattern-recognition receptors in the human myeloid compartment, yet its mode of action is poorly understood. TLR8 engages two distinct ligand binding sites to sense RNA degradation products, although it remains unclear how these ligands are formed in cellulo in the context of complex RNA molecule sensing. Here, we identified ...

|READ MORE|

carell_paper_245_550.100x0.jpg

Theories about the origin of life require chemical pathways that allow formation of life’s key building blocks under prebiotically plausible conditions. Complex molecules like RNA must have originated from small molecules whose reactivity was guided by physico-chemical processes. RNA is constructed from purine and pyrimidine nucleosides, both of which are ...

|READ MORE|

schneider_leonhardt_conradt_2019_wagner_550.100x0.jpg

The Fe(II) and 2-oxoglutarate-dependent oxygenase Alkb homologue 1 (Alkbh1) has been shown to act on a wide range of substrates, like DNA, tRNA and histones. Thereby different enzymatic activities have been identified including, among others, demethylation of N3-methylcytosine (m3C) in RNA- and single-stranded DNA oligonucleotides, demethylation of ...

|READ MORE|

cipsm_fest2019-1.100x0.jpg

The Scientific Oktoberfest will be this years meeting point for some of the internationally most distinguished researchers in the field of Life Science Chemistry & Biology.

The CIPSM Scientific Oktoberfest will be held from the 19th to the 20th of September at the Institute of Advanced Study IAS in Garching near Munich. At the two-day conference, researchers ...

|READ MORE|

tinnefeld-kramm_biorxiv_2019_550.100x0.jpg

The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor compound the fundamental core of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional intiation factor Bdp1, which is unique to the RNA polymerase (RNAP) III sytem, however, remained elusive. A poorly studied aspect in this context ...

|READ MORE|

jamchemsoc_141_2019_13643-13653_zinth_550.100x0.jpg

Psoralens are natural compounds that serve in the light dependent treatment of certain skin diseases (PUVA therapy). They are DNA intercalators that upon photoexcitation form adducts with thymine bases. For one psoralen derivative, 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT), the photoreactions are characterized here by nanosecond UV–vis and IR absorption ...

|READ MORE|

leonhardt_2019_mahamid_pnas_550.100x0.jpg

Lipid droplets (LDs) are ubiquitous organelles comprising a central hub for cellular lipid metabolism and trafficking. This role is tightly associated with their interactions with several cellular organelles. Here, we provide a systematic and quantitative structural description of LDs in their native state in HeLa cells enabled by cellular cryoelectron ...

|READ MORE|

leonhardt-2019_chiasson_scire_550.100x0.jpeg

Assembling composite DNA modules from custom DNA parts has become routine due to recent technological breakthroughs such as Golden Gate modular cloning. Using Golden Gate, one can efficiently assemble custom transcription units and piece units together to generate higher-order assemblies. Although Golden Gate cloning systems have been developed to assemble DNA ...

|READ MORE|

CIPSM Movie
LMUexcellent
TU München
MPG
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry