Multisubunit RNA Polymerases Melt Only a Single DNA Base Pair Downstream of the Active Site

J. Biol. Chem., 2007, 282, 21578-82 published on 27.07.2007
Journal of Biological Chemistry, online article
During gene transcription, RNA polymerase (Pol) II encounters obstacles, including lesions in the DNA template. Here, we review a recent structure–function analysis of Pol II transcribing DNA with a bulky photo-lesion in the template strand. The study provided the molecular basis for recognition of a damaged DNA by Pol II, which is the first step in transcription- coupled DNA repair (TCR). The results have general implications for damage recognition and the TCR mechanism.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry