Lys34 of translation elongation factor EF-P is hydroxylated by YfcM

Nature Chemical Biology, 2012, doi:10.1038/nchembio.1001, published on 17.06.2012

Nature Chemical Biology, online article

Lys34 of the conserved translation elongation factor P (EF-P) is post-translationally lysinylated by YjeK and YjeA—a modification that is critical for bacterial virulence. Here we show that the currently accepted Escherichia coli EF-P modification pathway is incomplete and lacks a final hydroxylation step mediated by YfcM, an enzyme distinct from deoxyhypusine hydroxylase that catalyzes the final maturation step of eukaryotic initiation factor 5A, the eukaryotic EF-P homolog.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry