Genome-associated RNA Polymerase II Includes the Dissociable Rpb4/7 Subcomplex*

Journal of Biological Chemistry, 2008, 10.1074/jbc.M803237200, 26423-26427 published on 30.07.2008
jbc, online article
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.  

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry