Rapid adaptive optical recovery of optimal resolution over large volumes

Nature Methods, 2014, doi:10.1038/nmeth.2925, 11, 625–628 published on 13.04.2014
Nature Methods, online article
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.  

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry