The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair

Chemical Communications, 2012, DOI: 10.1039/C2CC37735G, 49, 722-724 published on 28.11.2012

Chemical Communications, online article

The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(α-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.  

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry