Synthesis of 5-Hydroxymethyl-, 5-Formyl-, and 5-Carboxycytidine-triphosphates and Their Incorporation into Oligonucleotides by Polymerase Chain Reaction

Org. Lett., 2013, DOI: 10.1021/ol3033219, 15 (2), pp 366–369 published on 03.01.2013

Org.Lett., online article

The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readily accessible.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry