Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain

Angewandte Chemie, 2010, 49, 31, 5375 - 77 published on 19.07.2010

Angewandte Chemie, online article

LC-MS has allowed the amount of the post-replicatively formed DNA base 5-hydroxymethylcytosine (see picture; left) to be quantified in brain tissue. The nucleoside is most abundant in areas that are associated with higher cognitive functions, and its content in mouse hippocampi seems to increase with age. The new method enables hydroxymethylcytosine to be quantified with unprecedented accuracy.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry