Extending Polyketide Structural Diversity by Using Engineered Carboxylase/Reductase Enzymes

Angew. Chem. Int. Ed., Volume 55, Issue 3, Pages 858–860, DOI: 10.1002/anie.201510402
Angew. Chem. Int. Ed., online article

Enoyl-CoA carboxylase/reductases (ECRs) catalyze the selective α-carboxylation of α,β-unsaturated CoA-thioesters. Structure-based engineering of the active-site binding pocket of ECRs enabled significant alteration of their catalytic activity towards larger substrates. This facilitates the incorporation of unusual extender units into polyketide backbones, thus providing a novel method for the directed structural diversification of polyketides.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry