DMSO enhances electrospray response, boosting sensitivity of proteomic experiments

Nature Methods, 2013, doi:10.1038/nmeth.2610, 10, 989–991 published on 25.08.2013

Nature Methods, online article

We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry