Cleavable trifunctional biotin reagents for protein labelling, capture and release

Chem. Commun., 2013, DOI: 10.1039/C3CC42076K, published on 24.04.2013

Chem. Commun., online article

Trifunctional biotin reagents incorporating cleavable linkers are evaluated for their usage in protein enrichment. A linker based on the Dde protecting group leads to efficient release of protein targets under mild conditions. It additionally contains a masked trypsin cleavage site, which eliminates the majority of the tag during tryptic digestion.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry