A Photolabile Linker for the Mild and Selective Cleavage of Enriched Biomolecules from Solid Support

JOC, 2009, 74 Issue 21, 8476–9 published on 12.10.2009
JOC, online article
Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry