TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles

Org. Biomol. Chem., 2019, DOI: 10.1039/c9ob00508k
Org. Biomol. Chem., online article

We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody-fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.

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TU München
MPG
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry