Research Area D - Publications 2018

Epigenetics & Chromatin 2018, 11:41, https://doi.org/10.1186/s13072-018-0211-3
Epigenetics & Chromatin, online article

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Background
Epigenome-wide association studies (EWAS) based on human brain samples allow a deep and direct understanding of epigenetic dysregulation in Alzheimer’s disease (AD). However, strong variation of cell-type proportions across brain tissue samples represents a significant source of data noise. Here, we report the first EWAS based on sorted neuronal and ...

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PLoS ONE, 13(6): e0199782, https://doi.org/10.1371/journal.pone.0199782
PLoS ONE, online article

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Although distinct amino acid motifs containing consecutive prolines (polyP) cause ribosome stalling, which necessitates recruitment of the translation elongation factor P (EF-P), they occur strikingly often in bacterial proteomes. For example, polyP motifs are found in more than half of all histidine kinases in Escherichia coli K-12, which raises the question of ...

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Scientific Reports, volume 8, Article number: 8810 (2018), https://doi.org/10.1038/s41598-018-27125-6
Scientific Reports, online article

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Checkpoint molecules such as programmed death 1 (PD-1) dampen excessive T cell activation to preserve immune homeostasis. PD-1-specific monoclonal antibodies have revolutionized cancer therapy, as they reverse tumour-induced T cell exhaustion and restore CTL activity. Based on this success, deciphering underlying mechanisms of PD-1-mediated immune functions has ...

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Open Biol., 8: 180023, http://dx.doi.org/10.1098/rsob.180023
Open Biol., online article

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Every living cell possesses numerous transmembrane signalling systems that receive chemical and physical stimuli from the environment and transduce this information into an intracellular signal that triggers some form of cellular response. As unicellular organisms, bacteria require these systems for survival in rapidly changing environments. The receptors ...

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J. Bacteriol., vol. 200 no. 15 e00740-17, doi: 10.1128/JB.00740-17
J. Bacteriol., online article

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Many bacteria use extracellular signaling molecules to coordinate group behavior, a process referred to as quorum sensing (QS). However, some QS molecules are hydrophobic in character and are probably unable to diffuse across the bacterial cell envelope. How these molecules are disseminated between bacterial cells within a population is not yet fully understood. ...

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JCI Insight. 2018;3(5):e96322. https://doi.org/10.1172/jci.insight.96322
JCI Insight., online article

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Inflammatory bowel disease (IBD) is associated with enhanced levels of the IL-1 family cytokines IL-1β and IL-18, which are activated by the Nlrp3 inflammasome. Here, we investigated the role of inflammasome-driven cytokine release on T cell polarization and DC differentiation in steady state and T cell transfer colitis. In vitro and in vivo data showed that ...

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Life Science Alliance, Volume 1, No. 1, DOI: 10.26508/lsa.201800024
Life Science Alliance, online article

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The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the ...

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PLoS Comput Biol., 14(2), e1005987. https://doi.org/10.1371/journal.pcbi.1005987
PLoS Comput Biol., online article

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Translation of consecutive prolines causes ribosome stalling, which is alleviated but cannot be fully compensated by the elongation factor P. However, the presence of polyproline motifs in about one third of the E. coli proteins underlines their potential functional importance, which remains largely unexplored. We conducted an evolutionary analysis of polyproline ...

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Molecular Metabolism, Volume 9, March 2018, Pages 57-68, https://doi.org/10.1016/j.molmet.2018.01.011
Molecular Metabolism, online article

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Objective: Homozygous loss-of-function mutations in the gene coding for the homeobox transcription factor (TF) PDX1 leads to pancreatic agenesis, whereas heterozygous mutations can cause Maturity-Onset Diabetes of the Young 4 (MODY4). Although the function of Pdx1 is well studied in pre-clinical models during insulin-producing β-cell development and homeostasis, ...

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Mitochondrion, https://doi.org/10.1016/j.mito.2018.01.005
Mitochondrion, online ablage

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Mitochondrial localized proteins are mostly synthesized in the cytosol and translocated across the outer mitochondrial membrane via the translocase of the outer membrane (TOM) complex. Although the channel protein is conserved among eukaryotes, the receptor proteins are more divergent and show features specific to the plant lineage. OM64, which is a paralogue of ...

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Science, Vol. 359, Issue 6373, eaan1133, DOI: 10.1126/science.aan1133
Science, online article

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Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and ...

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Nature Communications, 9, Article number: 299, doi:10.1038/s41467-017-02582-1
Nature Communications, online article

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The ubiquitously expressed RNA-binding proteins Roquin-1 and Roquin-2 are essential for appropriate immune cell function and postnatal survival of mice. Roquin proteins repress target mRNAs by recognizing secondary structures in their 3′-UTRs and by inducing mRNA decay. However, it is unknown if other cellular proteins contribute to target control. To identify ...

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eLife 2018;7:e32010 doi: 10.7554/eLife.32010
eLife, online article

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The transcription factor c-Myc amplifies the transcription of many growth-related genes in cancer cells, but its role as an oncogene is not fully understood.
 

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Angew. Chem. Int. Ed., Volume 57, Issue 9, Pages 2314-2333, https://doi.org/10.1002/anie.201708459
Angew. Chem. Int. Ed., online article

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Nanobodies can be seen as next‐generation tools for the recognition and modulation of antigens that are inaccessible to conventional antibodies. Due to their compact structure and high stability, nanobodies see frequent usage in basic research, and their chemical functionalization opens the way towards promising diagnostic and therapeutic applications. In this ...

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