CIPSM - Research Area D

Research Area D - Publications 2021

Loss‑of‑function mutations in the histone methyltransferase EZH2 promote chemotherapy resistance in AML

12-Mar-2021

Julia M. Kempf, Sabrina Weser, Michael D. Bartoschek, Klaus H. Metzeler, Binje Vick, Tobias Herold, Kerstin Völse, Raphael Mattes, Manuela Scholz, Lucas E. Wange, Moreno Festini, Enes Ugur, Maike Roas, Oliver Weigert, Sebastian Bultmann, Heinrich Leonhardt, Gunnar Schotta, Wolfgang Hiddemann, Irmela Jeremias, Karsten Spiekermann

Sci Rep 11, 5838 (2021). https://doi.org/10.1038/s41598-021-84708-6
Scientific Reports, online article

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Chemotherapy resistance is the main impediment in the treatment of acute myeloid leukaemia (AML). Despite rapid advances, the various mechanisms inducing resistance development remain to be defined in detail. Here we report that loss-of-function mutations (LOF) in the histone methyltransferase EZH2 have the potential to confer resistance against the ...

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FUS-dependent liquid–liquid phase separation is important for DNA repair initiation

11-Mar-2021

Brunno R. Levone, Silvia C. Lenzken, Marco Antonaci, Andreas Maiser, Alexander Rapp, Francesca Conte, Stefan Reber, Jonas Mechtersheimer, Antonella E. Ronchi, Oliver Mühlemann, Heinrich Leonhardt, M. Cristina Cardoso, Marc-David Ruepp, Silvia M.L. Barabino

J. Cell Biol. 2021 Vol. 220 No. 5 e202008030, https://doi.org/10.1083/jcb.202008030
J. Cell Biol., online article

RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and DNA repair, and it undergoes reversible liquid–liquid phase separation (LLPS) in vitro. Here, we demonstrate that FUS-dependent LLPS is necessary for the initiation of the DDR. Using laser microirradiation in ...

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