Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

Nucleic Acids Resaerch, 2010, DOI: 10.1093/nar/gkq684, published on 21.07.2010
Nucleic Acids Research, online article
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.  

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry