Covalently circularized nanodiscs for studying membrane proteins and viral entry

Nature Methods, doi:10.1038/nmeth.4079
Nature Methods, online article

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

TU München
Helmholtz München
MPI of Neurobiology
MPI of Biochemistry