Research Area A - Publications 2017

Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1862, Issue 4, Pages 800-807, https://doi.org/10.1016/j.bbagen.2017.12.007
Biochimica et Biophysica Acta, online article

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Background

Recently diphenyl-pyrazole (DPP) compounds and especially anle138b were found to reduce the aggregation of α-synuclein or Tau protein in vitro as well as in a mouse model of neurodegenerative diseases [1,2]. Direct interaction of the DPPs with the fibrillar structure was identified by fluorescence spectroscopy. Thereby a strong dependence of the ...

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Nature, volume 552, pages 84–87, doi:10.1038/nature24650
Nature, online article

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DNA nanotechnology, in particular DNA origami, enables the bottom-up self-assembly of micrometre-scale, three-dimensional structures with nanometre-precise features. These structures are customizable in that they can be site-specifically functionalized or constructed to exhibit machine-like or logic-gating behavio. Their use has been limited to applications that ...

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Nature, volume 552, pages 78–83, doi:10.1038/nature24651
Nature, online article

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Natural biomolecular assemblies such as molecular motors, enzymes, viruses and subcellular structures often form by self-limiting hierarchical oligomerization of multiple subunits. Large structures can also assemble efficiently from a few components by combining hierarchical assembly and symmetry, a strategy exemplified by viral capsids. De novo protein design ...

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PNAS, October 3, 2017. 201704937, https://doi.org/10.1073/pnas.1704937114
PNAS, online article

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Green fluorescent protein (GFP) variants are widely used as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering their structure to develop in vivo optical sensors, such as for optogenetics and force transduction. Ensemble experiments have shown that the fluorescence of GFP is quenched upon denaturation. Here we study ...

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Scientific Reports,volume 7, Article number: 12276, doi:10.1038/s41598-017-12519-9
Scientific Reports, online article

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The antibody Fv module which binds antigen consists of the variable domains VL and VH. These exhibit a conserved ß-sheet structure and comprise highly variable loops (CDRs). Little is known about the contributions of the framework residues and CDRs to their association. We exchanged conserved interface residues as well as CDR loops and tested the effects on two ...

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Nature Methods, volume 14, pages 1090–1096 (2017), doi:10.1038/nmeth.4431
Nature Methods, online article

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Förster resonance energy transfer (FRET)-based tension sensor modules (TSMs) are available for investigating how distinct proteins bear mechanical forces in cells. Yet, forces in the single piconewton (pN) regime remain difficult to resolve, and tools for multiplexed tension sensing are lacking. Here, we report the generation and calibration of a genetically ...

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PNAS, vol. 114, no. 23, 6040–6045, www.pnas.org/cgi/doi/10.1073/pnas.1619843114
PNAS, online article

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Owing to the cooperativity of protein structures, it is often almost impossible to identify independent subunits, flexible regions, or hinges simply by visual inspection of static snapshots. Here, we use single-molecule force experiments and simulations to apply tension across the substrate binding domain (SBD) of heat shock protein 70 (Hsp70) to pinpoint ...

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ChemBioChem, Volume 18, Issue 13, Pages 1172–1176 , DOI: 10.1002/cbic.201600706
ChemBioChem, online article

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Mycobacterium tuberculosis (Mtb), the main causative agent of tuberculosis (Tb), has a complex cell envelope which forms an efficient barrier to antibiotics, thus contributing to the challenges of anti-tuberculosis therapy. However, the unique Mtb cell wall can be considered an advantage and be utilized to selectively label Mtb bacteria. Here we introduce three ...

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Science, Vol. 355, Issue 6331, eaam5488, DOI: 10.1126/science.aam5488
Science, online article

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Controlling the spatial arrangement of functional components in biological systems on the scale of higher-order macromolecular assemblies is an important goal in synthetic biology. Achieving this goal could yield new research tools and pave the way for interesting applications in health and biotechnology. DNA origami enables constructing arbitrary shapes on the ...

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J. Phys. Chem. Lett., 2017, 8 (7), pp 1585–1592, DOI: 10.1021/acs.jpclett.7b00371
J. Phys. Chem. Lett., online article

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Twisted intramolecular charge transfer (TICT) formation in hemithioindigo photoswitches has recently been reported and constitutes a second deexcitation pathway complementary to photoisomerization. Typically, this behavior is not found for this type of photoswitches, and it takes special geometric and electronic conditions to realize it. Here we present a ...

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ACS Nano, 11 (2), pp 1498–1508, DOI: 10.1021/acsnano.6b06812
ACS Nano, online article

 

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Advances in the engineering of nanoparticles (NPs), which represent particles of less than 100 nm in one external dimension, led to an increasing utilization of nanomaterials for biomedical purposes. A prerequisite for their use in diagnostic and therapeutic applications, however, is the targeted delivery to the site of injury. Interactions between blood-borne ...

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PLoS ONE 12(1): e0170625, doi:10.1371/journal.pone.0170625
PLOS ONE, online article

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Protein-protein docking protocols aim to predict the structures of protein-protein complexes based on the structure of individual partners. Docking protocols usually include several steps of sampling, clustering, refinement and re-scoring. The scoring step is one of the bottlenecks in the performance of many state-of-the-art protocols. The performance of scoring ...

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PNAS, vol. 114, no. 5, 1015–1020, https://doi.org/10.1073/pnas.1612681114
PNAS, online article

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Stable anchoring of titin within the muscle Z-disk is essential for preserving muscle integrity during passive stretching. One of the main candidates for anchoring titin in the Z-disk is the actin cross-linker α-actinin. The calmodulin-like domain of α-actinin binds to the Z-repeats of titin. However, the mechanical and kinetic properties of this important ...

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